Preparation of highly active alpha-chymotrypsin for catalysis in organic media.
نویسندگان
چکیده
A simple one step process for the preparation of free alpha-chymotrypsin, using an organic solvent to precipitate the enzyme from a buffered solution, followed by washing with organic solvents, is described. This preparation gave 132 times greater esterification activity than lyophilized powder.
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ورودعنوان ژورنال:
- Bioorganic & medicinal chemistry letters
دوره 14 9 شماره
صفحات -
تاریخ انتشار 2004